Supplementary MaterialsS1 Fig: Analysis of p53. RT-PCR (n = 3) as individual data points offered as mean SEM and b) Western Blot (n = 3) as built-in signal intensity normalized to the housekeeper GAPDH in A549 and A549rCDDP2000 cells, offered as mean SEM.(TIF) pone.0181081.s003.tif (278K) GUID:?14FDB30E-3983-43D0-A051-32D956FA1FFA S4 Fig: Analysis of p21. Analysis of p21 inside a) RT-PCR (n = 3) as AGN 205327 individual data points offered as mean SEM and b) Western Blot (n = 3) as integrated transmission intensity normalized to the housekeeper -actin in A549 and A549rCDDP2000 cells, offered as mean SEM.(TIF) pone.0181081.s004.tif (294K) GUID:?E24D6F54-09A2-4892-B280-230C3322DA5E S5 Fig: Analysis of SIP. Analysis of SIP inside a) RT-PCR (n = 3) as individual data points offered as mean SEM and b) Western Blot (n = 3) as integrated transmission intensity normalized to the housekeeper GAPDH in A549 and A549rCDDP2000 cells, offered as mean SEM.(TIF) pone.0181081.s005.tif (282K) GUID:?551131EB-B732-4C4D-8448-0538374DB9E1 S6 Fig: Analysis of XPC. PLS3 Analysis of XPC inside a) RT-PCR (n = 3) as individual data points offered as mean SEM and b) Western Blot (n = 3) as integrated transmission intensity normalized to the housekeeper GAPDH in A549 and A549rCDDP2000 cells, offered as mean SEM.(TIF) AGN 205327 pone.0181081.s006.tif (308K) GUID:?CD3A904C-C28C-4510-8855-15E0EB5946F7 S7 Fig: Analysis of GADD45a. Analysis of GADD45a inside a) RT-PCR (n = 3) as individual data points offered as mean SEM and b) Western Blot (n = 3) as integrated indication intensity normalized towards the housekeeper GAPDH in A549 and A549rCDDP2000 cells, provided as mean SEM.(TIF) pone.0181081.s007.tif (280K) GUID:?7FFD9F55-B256-495F-A3AC-E95649B41868 S8 Fig: Tables with individual data values. Desks with AGN 205327 specific data beliefs analysed for planning of all statistics within this manuscript.(DOCX) pone.0181081.s008.docx (96K) GUID:?3FC2ABB8-300E-498E-AAEE-B1B310E2C5C0 S9 Fig: Traditional western Blot of -Actin for p53 pATM and p21 normalization. Traditional western Blot of -Actin in A549rCDDP2000 and A549 cells. Lanes 1, 6, 11,: A549 untreated; lanes 2, 7, 12, A549 cells treated with 11 M cisplatin; lanes 3, 8, 13, A549rCDDP2000 untreated; lanes 4, 9, 14, A549rCDDP2000 treated with 11 M cisplatin; lanes 5, 10, 15, A549rCDDP2000 treated with 34 M cisplatin.(TIF) pone.0181081.s009.tif (76K) GUID:?B75769E7-2A3F-4EC5-B038-D242097C09DE S10 Fig: American blot p53. Traditional western Blot of p53 in A549rCDDP2000 and A549 cells. Lanes 1, 6, 11,: A549 untreated; lanes 2, 7, 12, A549 cells treated with 11 M cisplatin; lanes 3, 8, 13, A549rCDDP2000 untreated; lanes 4, 9, 14, A549rCDDP2000 treated with 11 M cisplatin; lanes 5, 10, 15, A549rCDDP2000 treated with 34 M cisplatin.(TIF) pone.0181081.s010.tif (58K) GUID:?9FE4A438-51DA-4677-B8A6-137CF0EFACF7 S11 Fig: Traditional western blot pATM. Traditional western Blot of pATM in A549rCDDP2000 and A549 cells. Lanes 1, 6, 11,: A549 untreated; lanes 2, 7, 12, A549 cells treated with 11 M cisplatin; lanes 3, 8, 13, A549rCDDP2000 untreated; lanes 4, 9, 14, A549rCDDP2000 treated with 11 M cisplatin; lanes 5, 10, 15, A549rCDDP2000 treated with 34 M cisplatin.(TIF) pone.0181081.s011.tif (90K) GUID:?73B764F1-DDAE-4A71-B609-D40194D8E309 S12 Fig: Western blot MDM2. American Blot of MDM2 in A549 (caption in dark words) and A549rCDDP2000 (caption in crimson words) cells displaying lanes of A549 untreated (caption ctrl), A549 treated with 11 M cisplatin (caption AGN 205327 11), A549rCDDP2000 untreated (caption ctrl), A549rCDDP2000 treated with 11 M cisplatin (caption 11) and A549rCDDP2000 treated with 34 M cisplatin (caption 34). Rings of MDM2 and GAPDH accordingly are labelled.(TIF) pone.0181081.s012.tif (1.3M) GUID:?297A69F7-F0FC-4E44-9870-6D5B635ECA13 S13 Fig: Traditional western blot p21. Traditional western Blot of p21 in A549rCDDP2000 and A549 cells. Lanes 1, 6, 11,: A549 untreated; lanes 2, 7, 12, A549 AGN 205327 cells treated with 11 M cisplatin; lanes 3, 8, 13, A549rCDDP2000 untreated; lanes 4, 9, 14, A549rCDDP2000 treated with 11 M cisplatin; lanes 5, 10, 15, A549rCDDP2000 treated with 34 M cisplatin.(TIF) pone.0181081.s013.tif (224K) GUID:?4054206A-F1D2-4710-AC3D-F1BDA054A79D S14 Fig: Traditional western blot SIP. American Blot of SIP in A549 (caption in dark words) and A549rCDDP2000 (caption in crimson words) cells displaying lanes of A549 untreated (caption ctrl), A549 treated with 11 M cisplatin (caption 11), A549rCDDP2000 untreated (caption ctrl), A549rCDDP2000 treated with 11 M cisplatin (caption 11) and A549rCDDP2000 treated with 34 M cisplatin (caption 34). Rings of SIP and GAPDH accordingly are labelled.(TIF) pone.0181081.s014.tif (2.0M) GUID:?A0EF879A-F123-4016-9D25-686EA0AADC77 S15 Fig: Traditional western blot XPC. American Blot of XPC in A549 (caption in dark words) and A549rCDDP2000 (caption in crimson.