Supplementary MaterialsAdditional file 1: Table S1. golf club and mucous cells. To facilitate the study of the biology of the human being SAE in health and disease, we immortalized and characterized a normal human being SAE basal cell collection. Methods Small airway basal cells were purified from brushed SAE of a healthy nonsmoker donor having a characteristic normal SAE transcriptome. The BC were immortalized by retrovirus-mediated telomerase reverse Pipemidic acid transcriptase (TERT) transduction and solitary cell drug selection. The producing cell collection (hSABCi-NS1.1) was characterized by RNAseq, TaqMan PCR, protein immunofluorescence, differentiation capacity on an?air-liquid interface (ALI) culture, transepithelial electrical resistance (TEER), airway region-associated features and response to genetic modification with SPDEF. Results The hSABCi-NS1.1 single-clone-derived cell collection continued to proliferate for ?200 doubling levels and? ?70 passages, continuing to keep up basal cell features (TP63+, KRT5+). When cultured on ALI, hSABCi-NS1.1 cells?consistently formed tight junctions and differentiated into ciliated, club (SCGB1A1+), mucous (MUC5AC+, MUC5B+), neuroendocrine (CHGA+), ionocyte (FOXI1+) and surfactant protein positive cells (SFTPA+, SFTPB+, SFTPD+), observations confirmed by RNAseq and TaqMan PCR. Annotation enrichment analysis showed that cilium and immunity were enriched in functions of the top-1500 up-regulated genes. RNAseq reads positioning corroborated manifestation of CD4, CD74 and MHC-II. Compared to the large airway cell collection BCi-NS1.1, differentiated of hSABCi-NS1.1 cells?on ALI were enriched with small EDNRB airway epithelial genes, including surfactant Pipemidic acid protein genes, LTF and small airway development relevant transcription factors NKX2C1, GATA6, SOX9, HOPX, ID2 and ETV5. Lentivirus-mediated manifestation of SPDEF in hSABCi-NS1.1 cells?induced secretory cell metaplasia, accompanied with characteristic COPD-associated SAE secretory cell changes, including up-regulation of MSMB, CEACAM5 and down-regulation of LTF. Conclusions The immortalized hSABCi-NS1.1 cell line has varied differentiation capacities and retains SAE features, which will be useful for understanding the biology of SAE, the pathogenesis of SAE-related diseases, and screening fresh pharmacologic agents. Electronic supplementary material The online version of this article (10.1186/s12931-019-1140-9) contains supplementary material, which is available to authorized users. value less than 0.05 was deemed significant. Results Generation of hSABCi-NS1.1 Based on our previous published sub-dataset [20], small airway epithelium has a different gene expression pattern than matched-tracheal and large airway epithelium from healthy nonsmokers (Fig.?1). For example, manifestation of SFTPB (surfactant protein), LTF (secretory cell gene) and small airway development-associated transcription factors GATA6 and SOX9 [24C27] are enriched in the small airway epithelium (Fig.?1). To ensure that the small airway epithelium recovered from your donor had standard SAE transcriptome, unsupervised clustering was carried out within the SAE transcriptome of the donor to compare with the previous small, large and trachea epithelium dataset. As expected, the microarray data of the donor clustered with the SAE samples when differential manifestation gene list of trachea vs small was assessed. Open in a separate windowpane Fig. 1 Standard small airway transcriptome features of the cell collection donors small airway epithelium (SAE). Data demonstrated is the unsupervised cluster analysis of microarray data from your cell collection donors small airway Pipemidic acid epithelium with data from previously published-microarray datasets that include 9 matched-trachea, large airway and small airway epithelium samples. Genes differentially indicated between Pipemidic acid the combined trachea and SAE (collapse changes ?2 fold, Benjamini-Hochberg corrected p? ?0.05) were selected to generate the plot. Examples of SAE-enriched genes (GATA6, SOX9, LTF and SFTPB) are indicated. The donors SAE clusters with the research SAE transcriptome, unique from your large airway and trachea epithelium After retro-hTERT genetic changes, the SABC were resistant to puromycin selection (Fig.?2a). The producing cell human population was a combined cell population termed as hSABCi-NS1. A single cell clone was isolated from hSABCi-NS1 (termed as hSABCi-NS1.1) (Fig.?2b). The heterogeneous morphology is likely because these cells were at different phases of the cell [22]. The hSABCi-NS1.1 clone survived and was expanded for more than 1?yhearing in vitro (Fig.?2c)The cells entered consistent growth after 200?days..